Primer+extension+analysis

=Primer extension analysis=

**Primer extension analysis** is a method used in transcript analysis to identify the start point of transcription.

The procedure is as follows:

**1.** Design a primer to hybridise to a known sequence within exon 1 of the transcript. Label the 5' end of the primer.

**2.** Extend the primer from its 3' end; extension will terminate at the 5' end of the mRNA template

**3.** The number of nucleotides between the 3' end of the primer and the 5' end of the transcript is unknown (//x// nt's)

**4.** Run three lanes in an electrophoresis gel: - Markers - Primer - Extended primer

**5.** The distance in the gel between the 'primer' band and the 'extended primer' band corresponds to //x// nts.