Immuno dot blot

An immuno dot blot is a method used to detect a particular protein in a heterogeneous protein extract mixture. Protein extracts from various tissues are placed in each of their own spots on a nitrocellulose membrane before being incubated with an antibody specific to the protein of interest. Excess antibody is washed off and the antibody visualised; the spot on the membrane which gives a signal is the tissue expressing the protein of interest.